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Equipment
XFe 96 Extracellular Flux Analyzer can simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate format, in real-time.
8 TSE Systems PhenoMaster mouse cages for metabolic, behavioural, and physiological monitoring.
Q Exactive Plus benchtop Orbitrap mass spectrometer, equipped with an Ion Max source and a HESI II probe (Thermo Scientific). This system is capable of measuring mass-to-charge ratios with less than 5 ppm error and a resolution of up to 140,000 and can also collect MS/MS fragmentation data. Mass spectrometer is coupled to a Dionex UltiMate 3000 ultra-high performance liquid chromatography (UPLC).
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