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Equipment

TSE LabMaster Automated Phenotyping system – Metabolic Cages

The TSE LabMaster Cages is a system for studying energy metabolism at whole animal level:

  • Automated metabolic and behavioural monitoring

  • Integrated system to study various aspects (feeding, drinking, locomotor activity, energy expenditure (gaz exchange: O2 consumption and CO2 production)

  • Uses standard Tecniplast cages

 

 

 

 

 

 

 

 

 

 

Cages and measurements characteristics:

  • Comparison of O2 and CO2 concentrations to the control empty cage every 6 minutes

  • Food and water weight recorded constantly

  • Every cage is equipped with a temperature sensor for enhanced measurement accuracy

  • Infrared beams for the activity recording in X and Y planes

 

What mice and for how long tested?

  • male mice (unless only female mice have the phenotype)

  • ~8-10 week old with body weight ~20 gram or above

  • Upto 8 mice can be tested simultaneously

  • For the indirect calorimetry and food intake the test lasts 7 days: 3 day as acclimation + 4 days of data collection

  • For the limited feeding or/and drinking experiments, testing of different diets etc. longer incubation could be previewed

It can simultaneously measure:

  • energy expenditure (O2/CO2, dO2/dCO2- difference in O2 and CO2 concentration values, VO2/VCO2 – O2 consumption and CO2 production, RER – respiratory exchange rate, H – heat production in kcal/h/kg) 

  • physical activity in meters

  • food/drink intake in grams or ml

  • perform food/water restriction experimenst.

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