Platform For Metabolic Analyses
Equipment
TSE LabMaster Automated Phenotyping system – Metabolic Cages
The TSE LabMaster Cages is a system for studying energy metabolism at whole animal level:
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Automated metabolic and behavioural monitoring
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Integrated system to study various aspects (feeding, drinking, locomotor activity, energy expenditure (gaz exchange: O2 consumption and CO2 production)
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Uses standard Tecniplast cages
Cages and measurements characteristics:
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Comparison of O2 and CO2 concentrations to the control empty cage every 6 minutes
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Food and water weight recorded constantly
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Every cage is equipped with a temperature sensor for enhanced measurement accuracy
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Infrared beams for the activity recording in X and Y planes
What mice and for how long tested?
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male mice (unless only female mice have the phenotype)
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~8-10 week old with body weight ~20 gram or above
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Upto 8 mice can be tested simultaneously
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For the indirect calorimetry and food intake the test lasts 7 days: 3 day as acclimation + 4 days of data collection
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For the limited feeding or/and drinking experiments, testing of different diets etc. longer incubation could be previewed
It can simultaneously measure:
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energy expenditure (O2/CO2, dO2/dCO2- difference in O2 and CO2 concentration values, VO2/VCO2 – O2 consumption and CO2 production, RER – respiratory exchange rate, H – heat production in kcal/h/kg)
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physical activity in meters
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food/drink intake in grams or ml
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perform food/water restriction experimenst.